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KMID : 0861020130280040083
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Korea Journal of Herbology
2013 Volume.28 No. 4 p.83 ~ p.92
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Anti-inflammatory effects of Lespedeza Cuneata in vivo and in vitro
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이호일:Lee Ho-il
황보민:Hwangbo Min/지선영:Jee Seon-Young/정지윤:Jung Ji-Yun/김영우:Kim Young-Woo/구세광:Ku Sae-Kwang
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Abstract
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Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute
mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation,
hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and
antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The
present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract
(LCE) in vivo and in vitro.
Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin E2 were detected
after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect
of LCE was determined in the carrageenan-induced paw edema model in rats.
Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin E2 (PGE2) via suppression
of iNOS and COX-2 expression. LCE inhibited the phosphorylation of IĸB indicating the suppression of
NF-ĸB pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by
carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory
cell infilterations.
Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in
LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of
anti-inflammatory use of Lespedeza Cuneata.
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KEYWORD
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Lespedeza Cuneata, anti-inflammation, NF-ĸB, LPS, Carrageenan
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